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lrp6  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc lrp6
    Lrp6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lrp6/pm41946692-267-98-101
    Average 86 stars, based on 1 article reviews
    lrp6 - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    other:

    Article Title: Development of a Chemical Probe to Enable Characterization of the Casein Kinase 1γ Subfamily.
    Article Snippet: The casein kinase 1γ (CK1γ) subfamily, while severely understudied, is implicated in diverse disease−relevant pathways, including WNT signaling and human cytomegalovirus (HCMV) replication.. While genetic tools exist to study CK1γ, the selective inhibition of CK1γ through pharmacological means remains underexplored.. Chemical probes, or potent and selective inhibitors, remain one of the most powerful pharmacological tools for uncovering protein biology.

    Article Title: FRZB-induced anti-angiogenic effect via Caveolin-1-mediated TGFβ signalling.
    Article Snippet: SDS PAGE and Western blotting Total protein was extracted from cells and tissues using Tortex lysis buffer (20 mM HEPES, pH 7.9, 350 mM NaCl, 20% v/v glycerol, 1% NP-40, 1 mM MgCl2, 0.5 mM EDTA, 0.1 mM EGTA) with freshly added protease inhibitor (Roche) and phosphatase inhibitor (Roche).

    Article Title: Development of a Chemical Probe to Enable Characterization of the Casein Kinase 1γ Subfamily
    Article Snippet: After overnight exposure to antibody, the blots were washed with Tris-buffered saline with Tween-20 (TBST), incubated for 1 h at r.t with secondary antibody, washed with TBST, and imaged after exposure to SuperSignal West Femto reagent (Thermo Fisher) for chemiluminescence.

    Article Title: Mast Cells Inhibit Stem Cell-driven Epithelial Repair in Inflammatory Bowel Disease via Suppressing Wnt/lrp6/β-Catenin Signaling Pathway
    Article Snippet: Equal amounts of colon samples from the rats and mice were lysed in radioimmunoprecipitation assay (RIPA) extraction buffer (C1035, Applygen) mixed with a protease inhibitor cocktail ( B14001 , Bimake), and then centrifuged at 12,000 rpm at 4°C for 10 minutes.

    Incubation:

    Article Title: Increased intake of saturated fatty acids during adolescence impacts the endoplasmic reticulum to induce insulin resistance and compromised bone mineral density ameliorated by Withaferin A.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Mast cells inhibit stem cell-driven epithelial repair in IBD via suppressing Wnt/lrp6/β-catenin signaling pathway.
    Article Snippet: .. Membranes were blocked with 5% (w/v) dried skim milk 676 (P0216, Applygen) at 22 °C for 1 h, and then incubated overnight at 4 °C with primary 677 antibodies against Lgr5 (1:1000, ab75850, Abcam), Lrp6 (1:1000, CST, #3395), and β-678 actin (1:1000, CST4967, Cell Signaling Technology). .. Next, membranes were incubated 679 with the corresponding secondary HRP-conjugated antibodies (ZB-2301, ZSGB-BIO) 680 for 1 h at room temperature and then visualized using Immobilon Western 681 Chemiluminescent HRP Substrate (Millipore Corporation, Burlington, MA, USA).



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    Santa Cruz Biotechnology codon
    (A) . Transwell analysis the migrated cells after TGF-β1 (5ng/mL), 74DHF (100nM), and VC (500μM) treatment. Scale bar: 100μm. The quantification of the stained cells was shown on the right (n = 3). (B) . Real time PCR of EMT marker Acta2 and Cdh1 expression in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) (n = 3). (C) WB analysis of α-SMA, CDH1, COL1A1 and <t>AKT1</t> in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) for 24 hours (n = 3). The grey intensity was quantified on the right. Data are presented as the means ± SEM compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. (*, P < 0.05, **, P < 0.01, ***, P < 0.001).
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    Image Search Results


    (A) . Transwell analysis the migrated cells after TGF-β1 (5ng/mL), 74DHF (100nM), and VC (500μM) treatment. Scale bar: 100μm. The quantification of the stained cells was shown on the right (n = 3). (B) . Real time PCR of EMT marker Acta2 and Cdh1 expression in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) (n = 3). (C) WB analysis of α-SMA, CDH1, COL1A1 and AKT1 in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) for 24 hours (n = 3). The grey intensity was quantified on the right. Data are presented as the means ± SEM compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. (*, P < 0.05, **, P < 0.01, ***, P < 0.001).

    Journal: PLOS One

    Article Title: Deciphering the synergistic mechanism of a novel flavonoid-antioxidant combination for asthma by combining systems pharmacology and experimental validation

    doi: 10.1371/journal.pone.0346165

    Figure Lengend Snippet: (A) . Transwell analysis the migrated cells after TGF-β1 (5ng/mL), 74DHF (100nM), and VC (500μM) treatment. Scale bar: 100μm. The quantification of the stained cells was shown on the right (n = 3). (B) . Real time PCR of EMT marker Acta2 and Cdh1 expression in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) (n = 3). (C) WB analysis of α-SMA, CDH1, COL1A1 and AKT1 in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) for 24 hours (n = 3). The grey intensity was quantified on the right. Data are presented as the means ± SEM compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. (*, P < 0.05, **, P < 0.01, ***, P < 0.001).

    Article Snippet: The membrane was incubated with primary antibody against α-SMA (Abcam, ab7817), CDH1 (CST, 1065), COL1A1 (CST, 3395), AKT1 (CST, 2938), and GAPDH (Abcam, ab70699) over night.

    Techniques: Staining, Real-time Polymerase Chain Reaction, Marker, Expressing, Comparison